Mono14: The pixel value is LSB aligned and found in bits 0–14. Bits 14 and 15 are always 0.

Mono16: The pixel value is LSB aligned and found in bits 0–14. Bits 14 and 15 are always 1.

Mono14 is the preferred and recommended pixel format to use. Mono16 is available because some client applications like MATLAB does not recognize the Mono14 format.

If you are using the Mono16 format, then you need to mask off bits 14 and 15.