Theoretically, the resolution you will get from a microscope is the camera pixel size divided by the microscope magnification.

So, for SC6000 with 25um pixel size the theoretical resolutions with microscopes are:
1x=25um
2.5x=10um
4x = 6.25um.

The reality is that you can't always reach these numbers because of manufacturing tolerances for the optics.  From a practical standpoint the more typical resolutions are:
1x=30um
2.5x=18um
4x=10um

As for all Thermography applications you don't want to be trying to measure the temperature of something that is about the size of one pixel.  You want to have several pixels so that you don't have any pixels on the edge.  Also keep in mind that the microscopes have different working distances.

1x=2.4in
2.5x=2.5x=1in
4x=0.5in

Although we can calibrate cameras with microscopes, the max temperature is generally lower because we don't want to damage the lens getting it too close to a hot source. 

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